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RNA for these 2 sgRNAs were in vitro transcribed, purified, and microinjected together with Cas9 into Pkd1 fl fl zygotes to generate Ddr1 Pkd1 f fll as previously described 18, 19. [url=http://buydoxycyclineon.com]doxycycline pronunciation[/url] In a recent study Rome et al.

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